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The Kennedy Institute has a state-of-the-art shared research facility to support single-cell and spatial genomics research.
Single-Cell Genomics: the facility provides best practise end-to-end workflows to assist with cell isolation, capture and library preparation. A cassette-based Sony SH800S FACS and LUNA-FX automated fluorescent cell counter are used for identification and isolation of live cell populations. For cell capture the facility is equipped with both the Chromium X and BD Rhapsody platforms. Library quality is assessed with an Agilent TapeStation prior to submission for sequencing at external services such as Novogene and Azenta. The facility is in routine use by Kennedy groups for multimodal single-cell profiling of gene expression, surface-protein abundance (CITE-seq) and T-cell and B-cell V(D)J sequencing in tissues of interest.
Spatial Transcriptomics: the facility offers a suite of complementary technologies for spatial transcriptomics. The Bruker GeoMx DSP platform allows researchers to use immunofluorescence microscopy to target regions of interest within tissues for targeted or transcriptome-wide profiling. The facility is also equipped with the Bruker CosMx platform which enables analysis of thousands of genes with effective segmentation at the single cell-level. The facility also supports use of the 10x Genomics Visium HD spatial workflows for whole transcriptome profiling of human and mouse tissues.
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